Preventing Laboratory Acquired Infections

Workplace-acquired infections are rare but still a possibility. In order for infection and disease to occur, there must be

  • an adequate number of organisms to cause disease (infectious dose), and

  • a route of entry to the body.

Knowing how infectious organisms are transmitted and what their infectious dose is, can help to evaluate the risk and avoid infection. Information about the organism should be gathered prior to working with it. Good starting points for gathering safety information about human pathogens are Safety Data Sheets (SDSs) and the Agent Summary Statements in the most recent edition of the Biosafety in Microbiological and Biomedical Laboratories publication.

Infectious agents are transmitted through one or more of the following routes of exposure:

  • Sharps injuries (needlesticks, cuts with contaminated broken glass, etc., also known as parenteral exposure)

  • Inhalation of aerosols (microscopic solid or liquid particles small enough to remain dispersed and suspended in air for long periods; about 5 micrometers or less in diameter)

  • Ingestion

  • Mucous membrane exposure (including the eyes, inside of the mouth and nose, and the genitals)

Using work practices that block routes of exposure can prevent workplace infection. Good microbiological techniques must always be used in the laboratory:

  • Eating, drinking, smoking, applying cosmetics or storing food for human consumption in laboratories is strictly prohibited.

  • Potentially contaminated hands should be kept away from the mouth, eyes, and non-intact skin.

  • Hands should be washed frequently, even after wearing gloves, and scrubbed vigorously with soap and water for a full 30 seconds (as long as it takes to sing "Happy Birthday" or the "Cyclone Fight Song"). The physical removal of organisms from the skin is just as important as using a disinfectant.

  • Work surfaces and equipment must be decontaminated immediately after using biohazardous materials.

  • Wearing appropriate personal protective equipment (PPE) blocks potential routes of exposure.