Centrifuging

Use the following techniques to prevent aerisolization and spills when using a centrifuge:

• Sealed tubes and safety buckets that seal with O-rings should be used. To avoid spills from broken tubes, inspect all tubes, O-rings, and buckets for damage before each use.

• Leaks can be prevented by not overfilling centrifuge tubes. The outside of the tubes should be wiped with disinfectant after they are filled and sealed.

• Rotors and centrifuge tubes should be opened inside a biosafety cabinet. If a biosafety cabinet is not available, a minimum of ten minutes settling time should be allowed before opening.